I was happily absorbed in my slightly vegetative stupor on the couch when my roommate walked into the room and starting talking about physics. Ugh, physics, I thought, but I politely listened as she began talking about lenses, specifically how they are related to sight. It is common knowledge that the images we see are inverted on the retina, and then further processed. However, my roommate was discussing experiments done on humans that inverted their vision by 180 degrees and found that, though at first they could not function normally, eventually they adapted. I thought this was fascinating, and wondered what the brain had to do with this process. Unfortunately my roommate’s knowledge was pretty limited, so I decided to do some research of my own.

Research on visual distortion of the retina has been going on for quite some time. Devices have been used that invert the retinal image, so that everything is seen upside down. At first subjects wearing this device will reach for things and miss, or will bump into things as they travel about a space. Eventually, they adapt. What I wanted to know is how do they adapt? What changes take place in the brain that allow them to do so? Is it just simply learning to reach a little farther to grab something, or walk a bit differently to avoid bumping into something? What is happening at the neural level?

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The good Lynch of Stranger Fruit was honored as the CASE/Carnegie Professor of the Year awardee for Arizona. Everyone go applaud!

This week I’ve been diving in a little more into doing some actual research myself. Nothing breakthrough mind you, just some simple experiment to sort of understand the world around and inside of me a little better. My partner and I are looking into how the optic nerve develops inside of zebra fish and how its development may be affected by developing the fish in total darkness. We are trying to stain 1-2 day old zebra fish with Dye-I by simply poking the dye into the retina with any sort of small sharp object we can find. We’ll then separate the groups of stained fish into those that develop in a small flask with normal exposure to light (about 10-15 hours a day of UV light) and those that develop in a tin-foil-wrapped flask with no UV exposure in which feeding will be done under red light.

Every few days, I hope to take some of the fish out and look at how their brains are developing using a fluorescent microscope that allows me to see how the dye is traveling. With any luck, I’ll see a decently clear pattern of paths of nerves from the retina to the lateral geniculate. I’m not sure where it leads from there as the zebra fish has mainly the midbrain rather than our large forebrain with a thalamus and cerebral cortex. More research on my fish is definitely needed before I can do any real analyzing of the staining technique, but my real problem right now is just getting some fish stained!

Using a pin head dipped in dye, I had been trying to poke the retinas of these fish. Once the retina ruptures, almost any amount of dye should stain the retina and lead to further staining of the optic nerve as development continues (from what I’ve read, usually the rods and cones develop about 4 days at the earliest). I ran into many problems with this of course and have been fishing (oh so hilarious, I know) for new solutions ever since.

The first problem I had is that the head of the pin is about the size of the fish’s eye itself, so trying to rupture the eye with it is like trying to shove a baseball bat through my own. Of course actually rupturing the retina with this gigantic tool will only happen if my mounted fish would stop moving every time I get close to it, which is the second problem. So far, I’ve created new tools by heating TLC spotters over a flame in the organic chemistry lab and then pulling it in half so that the glass is pulled into a tiny pipette with a head just smaller than the fish’s retina. This has been successful after coupling it with a technique for knocking out my fish to keep them still. I burst a small bubble of anesthetic (0.5 MESAB) over the water that I’m using to mount my fish before injecting the fish in augar onto the slide. This does a great job in keeping the fish still so I can do my dirty work of staining its eye, but sometimes the concentration of anesthetic is too strong and the fish will die. All in all, with much more research and some patience this may turn out to be a fun little project. If you have any ideas as to where I should be looking for research and anything else I could be doing with this experiment, let me know.
~Bright Lights

We’ve been talking quite a bit about how information is processed in our brains so that a specific reflexes and cognitive actions can be produced. It’s also the end of the cross country season and my mind has been mixing the two. Take Steve Prefontaine (one of the greatest American long distance runners of all time) for example. I was watching a video clip of Prefontaine running and paused it right as he was putting his foot down and picking his other leg up. He doesn’t extend his leg out very far. Instead, he lifts his knee up so that he can drive his pronated foot into the ground just under his hip. Since his foot is already pronated, he can further drive his leg behind him and use the ball of his foot as a launch pad to drive himself forward. This technique saves a lot of energy and keeps the runner mostly in the air, rather than on the ground (which is what you want).

Sadly, no one brought the technique to my attention until about halfway through college. I’ve been overextending my legs without picking up my knees, landing on my heels, and rolling to the balls of my feet. This means I use more energy and a lot more time in projecting myself forward each stride. Ever since I learned of proper running techniques I’ve been trying to make them an automatic reflex in my strides, but this is incredibly hard to do.

For the past twenty two years, neurons in the motor cortex of my frontal cortex, cerebellum, Thalamus, and several other regions of my brain have been adapting to coordinate specific muscle actions to create my presently crappy running technique. This spider web of nervous tissue is constantly changing as neurons diverge and converge on each other, become more sensitive to or produce higher concentrations of specific neurotransmitters, and develop other specific interactions with other neurons that perfectly coordinate my behavior and actions. However, it has taken twenty two years to perfectly coordinate all this interaction so that I can run so terribly, meaning that learning to truly run could take a long time as I prune and pair more neurons to coordinate a totally different reflex.

So far I’ve been trying to reshape my neural network by doing one legged hang cleans. In the exercise I have to shrug a large amount of weight off one leg in a lunging position, get under the weight in mid air and catch it as it comes down in the same position. It is difficult to catch the weight without landing on the ball of my foot, which is pleasantly placed directly under my hips, making my body develop the correct foot placement as a reflex. This learning is actually the reshaping of the network of neurons inside my brain that deal with motor coordination as they make new interactions, destroy old ones, change their amplification of signals, or change their functions completely. At any rate, I haven’t even scratched the surface of how intertwined the processes are that go inside this skull of mine to create who I am and the things that I do. But I do find the little I know pretty amazing, and can only hope that my non-declarative memory will eventually kick in.
To see the proper running form, take a look at Michael Johnson in his world record 200m race.at the 1996 olympics.

I found an article about new brain cells that I thought was really interesting. Researchers at the Yale School of Medicine discovered the mechanism behind how new neural cells are integrated into the adult brain. It turns out that new neural cells take a while to mature and fully integrate themselves into existing neural networks in the brain. While they are maturing, they rely on signals from other brain regions so that they do not disturb ongoing functions of the brain. They can receive input from these other regions for up to 10 days before they are ready to make any of their own outputs. So how long does it take to fully develop their synaptic connections so that they can talk to one another? Up to 3 weeks.

So why do we care; what is significant about this discovery? This mechanism sheds light on how neural cells integrate themselves into existing networks, which will impact how stem cells are used to replace neurons lost to injury or disease. The main concern is about neurons firing inappropriately, which could cause seizures or cognitive dysfunction.

The full article can be found in the Journal of Neuroscience, Vol. 27

This is the last time I’ll pester you, I promise. The DonorsChoose challenge ends after the end of this month, and we’ve done well. We met my goal of raising $20,000 dollars, 200 freethinkers have stepped up to make donations, and 30 of my 31 chosen projects have been fully funded. That does mean that there is one project that isn’t quite there yet: Embryology in the Classroom is $292 shy of completion. If a few more could chip in a few more dollars, we can achieve perfection.

Good work, everyone!

Hello again, it’s been a while so I thought I’d drop in a comment or two about what I’ve found recently in the news about neurobio. I’ve lately been reading about neurotransmitters and how they bind to sites in specific neurons, instigating depolarization across the membrane of the neuron and allowing for an action potential to communicate to hundreds of thousands of other neurons. This communication between neurons in the central nervous system is relayed into actions in the peripheral nervous system resulting in behavior. But how is this synchronized? What neuron does what? What must be connected to what and why? These are all questions that may take a while to be answered, but we are finding new developments everyday.

In an article from Cornell News, I read about an experiment by James Goodson and Andrew Bass (2000) in which neurotransmitters’ role in the display of sex characteristics in plainfin midshipman fish were examined. In this particular fish, males will make vocal calls through the water that attract females who will come to the site to lay eggs for the vocalizing male to fertilize. However, a second type of male that is unable to make vocal calls waits nearby so that once the eggs are laid, he can get some free-fertilization-action.

Goodson and Bass anesthetized and stimulated the anterior portion of the hypothalamus in each fish to stimulate either a vocal call, or the female’s short grunt (a response to the male’s call). After stimulating normal calls in each fish, the neurotransmitters isotocin and vasotocin (identical to the mammalian oxytocin and vasopressin) were administered to the anterior hypothalamus of each fish. When administered, fish that normally could make calls lost the ability to do so and developed female like grunts, similar to the type II males that could not call but rather grunted like females. This meant that a trait that was typically thought to be controlled by sex (controlled or linked by the gonads) was actually independent, and regulated completely by the brain.

Who knows how many of our traits are linked to gonad development, probably much fewer than we might originally think. If I was given a good dose of estrogen would I not want to play football or wrestle with my best friends?…doubtful (it might just turn into flag football with the Vikes or a pillow fight). At any rate, we shouldn’t be so quick to make judgment calls on biology’s effects in gender behavior.

If you’re still interested in weighing in on whether the academic meetings in San Diego should go on in the wake of fires in Southern California, Terra Sigillata has a thread on that very subject.

I promise the small meeting I’m planning to attend won’t clog up the freeways.