If you want to raise hordes of zebrafish…

…like I do, here’s a useful resource: Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction. It’s text and a video guide to familiar procedures.

Unfortunately, it also assumes you have a commercial zebrafish rack, which if you buy the smallest size available, will set you back about $10,000. I’ve just been using an array of 5 gallon tanks, each with its own filter system, which is cheap to set up, but a pain to maintain. I’m going to bite the bullet and build my own DIY flow-through system this summer, though, which I expect will cost a few hundred dollars…which is more in my budget. If you want to follow along, here’s a paper on a Modular, Easy-to-Assemble, Low-Cost Zebrafish Facility — it’ll be something like that. It’s not exactly the kind of show aquarium you’d have in your home, but if all you care about is embryo throughput, it’s loads of fun and lower maintenance than what I’ve been doing so far.

Did a bat land on you at the Kelso Depot in the Mojave Desert?

Boosting signal on this, because it’s potentially very urgent and the person at risk could be anywhere in the world at this point.

A week ago, on April 30, a visitor to the Kelso Depot in the Mojave National Preserve had a bat land on his neck. The bat — a Myotis lucifugus a.k.a. little brown bat — has since tested positive for rabies, and now San Bernardino County officials are trying to find the man. They don’t know if he was bitten or scratched: it wouldn’t take much of a bite to transmit the disease, and if that happened he’s got to get vaxxed.

Details on the incident and public health contact info are here. The guy doesn’t have a lot of slack before getting to the doctor at this point: onset of symptoms can start mere days after a bite. (Or years, which has caused people to falsely assume they’ve dodged a bullet.) Before symptoms start prevention is straightforward and no longer arduous. I’ve had rabies shots and they weren’t the worst injections I had that year. (Individual mileage varies there, but they’re way better than they were back in the day. Mine were a breeze.)

And it’s a good opportunity to remind people in bat and rabies country that while transmission of rabies from bats to humans is quite rare, bats exhibiting unusual behavior (like not being shy or nocturnal) should be given a wide berth and reported to local authorities.

But what about capitalism, Rush?

Rush Limbaugh’s network has lost millions of dollars this past quarter, and he may be on the way out. Apparently, the problem is that advertisers have fled his show in droves, especially after his rages against Sandra Fluke. But you knew he’d have an excuse: it’s all the women’s fault.

Despite sources close to Limbaugh that accuse Dickey of scapegoating the radio host for a bad quarter, Limbaugh himself has addressed his advertiser woes in the past. But Limbaugh doesn’t see his offensive bloviating as the problem driving mainstream advertisers away; instead, he accuses media buyers who are ”young women fresh out of college” and “liberal feminists who hate conservatism” of “trying to harm” him.

I had no idea that women now controlled all the media; did you women reading this know you had such immense power?

Now I have a few requests. Right after you’re done flushing Limbaugh’s career down the toilet, could you shut down Fox News and Glenn Beck (well, you’ve been doing a good job on him so far), and perhaps redirect some small fraction of those advertising dollars to Freethoughtblogs.com? Thanks, much appreciated.

The first day of the rest of my summer!

twitch

It’s going to be a good season, I can tell already. It’s finals week, so I’ll still have an abrupt pile of grading to do on Thursday, but otherwise, my teaching obligations are done for the semester. Now I’m trapped, trapped I tell you, in Morris for almost (I do have two quick trips to Europe planned) the entire summer with a collection of administrative responsibilities, but the good part of that is that I have ambitious plans for what I’ll be doing in the lab. I’m also going to be living the good life.

So this morning I slept in to 7:00. I know, it’s slothful of me, but I have the freedom to indulge myself a little bit now and then. After I got up, I took a nice brisk walk downtown, did some shopping, stocked up on some fresh vegetables, and once I got home, chopped them up and set them to soak in a tasty marinade. I’ll roast them up for dinner tonight.

Then I started reading up an accumulated mass of papers that’ll give me some implementation ideas for the work I have planned.

I’ll have a student working with me, and we’ve got a couple of projects in the works.

  1. There’s some boring scut work to be done: lab cleanup, clearing out old reagents from the refrigerator, making up new stock solutions. Don’t be disillusioned, but part of the research life is janitorial…so much dishwashing.

  2. My grand plan requires an expansion of my fish colony to include multiple genetic strains, so we’re going to be scrubbing tanks, sterilizing surfaces, setting up new tanks with boring feeder fish to get the nitrogen cycle going and condition the water, getting the brine shrimp hatchery (live fish food!) thriving, all that sort of stuff that qualifies you to be a clerk in a pet store.

  3. Once all the tanks are bubbling away happily, we’re getting some new strains from the zebrafish stock center. Then it’s a few months of nursing them along, collecting eggs, propagating new generations and raising them to adulthood to get the whole colony self-sustaining, and to prepare for crosses to produce hybrid strains. After all this, my student will be well-trained to be a hobbyist aquarist.

  4. Concurrently, we’ll be doing some real science on the embryos we get, analyzing their behavior quantitatively to identify consistent differences between strains, and also in response to different environmental stresses. This is going to require a bit of computer work and — oh, no! — basic math to develop image analysis protocols. That’s what I’ve been reading about; I’ve done some of this in the past on an obsolete software system, so I’m going to have to piece together some custom bits to make it all work. I’ve been reading about Fourier analysis and power spectra all morning, and I’m kinda jazzed. Math! Computers! Embryos! Science!

  5. The dream is that once we’ve found some subtle differences between different strains, we can start doing crosses to dissect out and isolate the genetic components, if any, of the behavior. That’s going to take a couple of generations of crosses, which means that if I’m lucky we’ll get those results next year, or at worst, the year after. Behavioral Genetics! Yay! Long generation times! Boo!

It’s step #4 that’ll give us some quick quantitative results, I hope, and maybe something presentable at a meeting or even publishable. It’s all going to be preliminary and descriptive, but that’s what you need to do to establish a foundation for experiments.

Unfortunately for you, I won’t be blogging about any of the details of the work this summer — I’ve been scooped before when I foolishly posted protocols on the web, and especially when you have a very small lab with limited humanpower to throw at a problem, that costs. But I might just occasionally say a few general things about the kinds of analyses we’re doing.

Or I could talk about the moldy stuff we throw out of the refrigerator. That’s probably safe.

“…a pretty little white girl ran into a black man’s arms…something is wrong here”

It was a horrific story out of Cleveland: three brothers have been arrested for imprisoning three women for over a decade in their home. I can’t even imagine the nightmare those women lived through.

Charles Ramsey, the neighbor who helped break them out, at least provides a little light-hearted relief.

And right now we’re starting to see pictures of tearful reunions with family members who thought the missing women were lost forever.

amandaberry

But let’s not forget, this isn’t all about happy outcomes. Those women had years of their young lives stolen from them, were locked away from the world, and had many losses — Amanda Berry’s mother died 3 years after she was kidnapped. None of that is coming back.

What the hell was wrong with those three men that they could do this? Did they just see those women as toys to be possessed, with no empathy and no human feeling of any kind for what they did to them?

As has been noted in the comments, Sylvia Browne ‘predicted’ that Amanda Berry was dead and “in the water” in 2004. She said that to her mother on the Montel Williams show!

Browne is an evil amoral fraud, and Montel Williams is a repulsive enabler.

The tent metaphor gets a workout

Ophelia reports that CFI, at least, has the right idea.

ANY large group who feels like they have a particular beef with religion (or pseudoscience, or other wacky beliefs) has a legitimate interest in addressing that problem as a group.

At CFI-L.A., we’ve hosted Black Skeptics, Spanish-speaking atheists, gay and lesbian humanists, and others who’ve had specific troubles in our society based on who they fundamentally are. And I say, welcome to our tent.

Ideally, our whole movement is a coalition of individuals and groups who all have an interest in promoting a secular and reason-based society. And if some of those groups want to get together to fine-tune their methods for dealing with and changing this uber-religious society we live in, more power to them.

How can we help?

That’s the way any inclusive organization ought to be: welcome to our tent. Hey, can you help us make our tent bigger? Is there any particular patch of ground we ought to expand into? It also echoes our sentiments exactly when we set up Freethoughtblogs — we want to increase the range of voices speaking for this cause.

If you’re looking for friends, it’s really easy to choose between tent-makers and tent-nazis.

Mary’s Monday Metazoan: More charismatic megafauna?

Oh, no. The metazoan curator sent me this photo for this time around, and I groaned a bit: more big furries. I told her, “Where are the tubeworms, the crustaceans, the zooplankton? Why no jellyfish or echinoderms?” And she said, “But they’re so cute!” and gave me that look. That look that means I have to do as I’m told.

kangaroo-and-wombat

You know, if she starts sending me cats, I’m just gonna blow up. Me posting cats? That would be one of the signs of the End Times, along with the Last Trump and deluges of blood and whores riding dragons.

(via NatGeo)