Spider update (no spider photos)

I’m getting a little anxious — my spider family is in a quiet phase right now. I have 6 breeding pairs of adults (well, Gwyneth ate her consort after mating, so 5½, and maybe bred pairs is the better term). I’m down to one egg sac — again, from Gwyneth, who is a sick Goth freak because she knitted an ugly, sloppy sac with the dead corpses of her prey imbedded in it, but it does have developing embryos inside it. She also littered the floor with decapitated fly heads. Gwyneth scares me sometimes.

But otherwise, I’m just waiting for them to produce more. My goal is to have a steady reliable output of eggs, and these little hiatuses are nerve wracking, but also understandable, since the colony is so small yet.

I do have a lot of tiny little juveniles coming up, at least. They’re getting a little overwhelming — these are my spider-children, in these little vials I picked up at JoAnn Fabrics (they’re intended for storing and sorting beads, but I have perverted them to my own wicked ends.) Thirty vials, thirty hungry little babies.

I have to go in every couple of days and tend to them. Put one or two flies in each vial (I made a little fly-shaker out of an Eppendorf tube — it’s like a salt shaker, only when you shake it flies come out), give ’em a spritz of water from an atomizer, and agonize over their health and predatory instincts. As they get big enough, I move them to an adult-sized tube, and when I’m confident of their sexual maturity, I’ll pair them up.

But right now it’s a waiting game with placid little beasties (except for Gwyneth) quietly tending to their webs, nibbling on flies and crickets, making me fret over when they’re going to spawn again.

By the way, over half the vials I’m cultivating contain Gwyneth’s progeny — she’s a fecund little monster. I’ll be interested to see if her distinctive behaviors carry on into the next generation. I’m planning on doing some inbreeding of her offspring to see if I can get a brood of savage spider mothers.

Spider update (no photos)

We’re in a mundane phase of this project — I’ve got swarms of baby spiders, a handful of wild-caught adults, and I’m waiting for them to reach sexual maturity, so I can start breeding lab lines.

I’ve got a naming convention — the wild-caught adults (Generation 1) all get simple names like Cathy, Barney, Gwyneth, etc. I’ve had a few of them die off already, although I think it was actually murder. It seems that crickets above a certain size are actually able to turn the tables and eat the spider, or at least kill it, and they will definitely consume an egg sac if they stumble on it. I’m learning lessons as I go along — only small crickets. I’m considering trying mealworms as a safer alternative.

For the second generation, each clutch gets named by the first letter of their mother’s name, and the month their egg sac was made. As they reach sexual maturity, they’ll get a letter after the month to distinguish them as individuals. Third generation will get the initials of both parents, but we’re not up to that point yet. I am planning to keep track of the pedigrees of these spiders as I go, in case something unique and interesting crops up.

I do have a sad story. I’ve been particularly watching on individual, GIIXa, Gwyneth’s daughter by an unknown father, laid in August. I’d been calling her Igor, because she had a few deformities — her left foreleg was much longer than her right (it looked like a duplication of one limb segment), and her two hindlegs had limited mobility, so she crawled around dragging her hindlegs, and with her left foreleg raised high up in the air. She made it to near-adulthood, so clearly she was able to capture and eat flies, but today I found that she had died at last. I’d actually be interested in teratological defects, and that I’ve already seen one isn’t too surprising, given how prolific the spiders are.

I’m also pretty sure some of the second generation are reaching sexual maturity, which is about right, since some of them are almost two months old. I’ve got one, AIIXa (a son of Amanda), which already has the massive dark pedipalps that allowed me to recognize him as male — he’ll be losing his virginity soon. I don’t want to give him to one of the first generation females, since they’re so much bigger they might just eat him, but am waiting to be confident that one of the second generation females is ready.

I’m a little bit nervous about getting this next generation to maturity, because I’ve noticed that this species has become scarce as the weather is changing. We noticed that the best spots for finding them this past summer were our garage and sun room, places with lots of fresh air (and diverse prey, I presume) that were still sheltered by the proximity of a human habitat. We couldn’t find any indoors, but only in these attached spaces. Now we’re only finding Pholcidae out in the garage, as if there has been a seasonal shift in the spider populations. It’ll be interesting to see what spider species survive a Minnesota January. Maybe Steatoda/Parasteatoda are moving indoors? Maybe they die off and leave behind egg sacs to weather the winter and emerge in the spring? I’ve got my eye on a couple of egg sacs attached to my garage door, and I may bring them into the warm to see if they hatch out.

Anyway, that’s all I’m doing right now, the tedious business of spider breeding. I’ve ordered some of the reagents I’ll need to start poking around spider embryos, but those won’t arrive until next month, and I’m not doing experiments on babies until I have a stable colony anyway.

Spider party!

Just another mundane spider update. This batch of babies are now 18 days post-fertilization, and they’re just rockin’ out in their dish.

I also got some good news: I was awarded a small in-house grant to pick up a bunch of supplies for embryo imaging, so that’s in the works. I ordered a few necessities today.

And now everywhere I go on the internet, ads for halocarbon oil pop up everywhere. Or does everyone get those?

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Betty was HUNGRY!

It’s October. That means I’ve got free rein to post horrifying spider videos, right?

I’ve got a feeding schedule for my colony, so every Monday and Thursday I open up the incubator and fling a bunch of living, walking, wingless Drosophila into every spider tube.

Today is Monday.

Now usually, the spiders sit unperturbed by the intrusion of insects into their domain, and they’ll just watch and wait, and the next day I find the withered corpses of their prey in their webs. Today, I guess Betty was hungry, because she leapt unto one of the hapless flies within seconds of it landing on the web. She was so fast she had it trussed like a Christmas turkey before I could get her under a camera.

I got a bit of the aftermath in a video, at least. It’s below the fold. It’s probably not a good one for the arachnophobes to watch.

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You know, I’m not going to stop making these spider videos, because they’re awesome

I’ve got these spider babies that I now know are exactly 8 days old after they were laid in their mamma’s egg sac, and we’re seeing the transition from spherical egg to lightly sculpted leggy thing wrapped around a spherical ball. So I took some pictures. I also tried putting them on my compound scope and seeing if I could visualize cells in the tissue — it didn’t work. The spider embryos are thick and round and opaque, and further, I was just looking at them dry — I’ve got to work on getting them in a better medium and improving the optics.

Also, the more bloodthirsty of my followers have asked me to catch the babies in the act of feeding, so just to appease them (please don’t hurt me!), I’ve also included a short clip of what happens when I dump a bunch of flies into a tube of baby spiders.

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Panic in Spider City

I didn’t update yesterday, and nothing much today either, because I’m new to this spider business and have lots to learn — like planning ahead. I’ve got all these vials full of spider babies right now, and they have eaten all of my flies, every one. I set up four more bottles of flies a bit more than a week ago, and they’re at the stage where I’ve got lots of pupae but the adults haven’t eclosed yet, which should happen any day now. But it means my babies are hungry right now, and I’ve got nothing to give them.

I’m a bad spider daddy.

I set up a bunch more fly bottles today and will start staggering production every 3 or 4 days, but wow, when you’ve got a few hundred spiderlings, the logistics of keeping them supplied with flies is a little more involved than I expected. Also, I don’t quite have the rhythm yet. The goal is to raise just enough to maintain a small colony at a stable productive size, and right now I’m producing to excess because I’m uncertain about mortality and how quickly they’ll be consistently reproducing. At a guess, they reproduce a lot faster than I expected!