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Dec 05 2011

Techniques to go with the tools

So you read that cool summary of how to build a molecular biology lab for $500. But wait, you don’t know what you’d do with the mobio toys! Here’s how to correct that: go to a workshop.

THE MICHAEL SMITH LABS AND ADVANCED MOLECULAR BIOLOGY LAB PRESENTS OUR MOLECULAR BIOLOGY WORKSHOPS 2012 WINTER/SPRING Session.

ONE WEEK VERSION (5 DAYS) – MOLECULAR TECHNIQUES WORKSHOPS
FEB 13 – 17, 2012 (CAN$1400)
DESCRIPTION: Recently updated: This intense 5 day workshop will focus on a myriad of different techniques used in the molecular manipulation of DNA, RNA and protein, as well as inclusion of exercises in some basic bioinformatics tools. Primarily aimed at researchers who are new to the area, familiar but require a quick updating, or would like more practical bench training.

PHILOSOPHY: Whilst molecular techniques have evolved at a speedy rate over the last few decades, the underlying biochemical principles behind the vast majority of them has actually changed little. This workshop therefore combines opportunities to perform the latest, as well as commonly used older techniques, with particular attention to the chemical nuts and bolts behind them. In all, this allows the researcher to not only gain needed familiarity with the techniques, but also achieve a comfortable theoretical level to allow for both (1) that all important skill of troubleshooting, and (2) the often undervalued skill of judging the utility of “tricks” that aim to speed up, or lower costs of a given methodology.

TECHNIQUES COVERED: Various nucleic acid purification methodologies (silica bead, organic, and/or pI based), restriction digests, ligations, dephosphorylation assays, agarose gel electrophoresis, transformation (including electroporation), PCR, reverse transcriptase assay, real time qPCR, basic bioinformatics, (including blast tools), SDS-PAGE, Western blot analysis, Isoelectric focusing strips, and 2D protein gels.

Full details can be found at http://www.bioteach.ubc.ca/mb-workshops/#molecular

See? You can become a mad scientist for cheap nowadays.

(Also on Sb)

18 comments

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  1. 1
    Stevarious, Public Health Problem

    Aw man even more reasons to finish my Dr. Horrible costume!

  2. 2
    madtom1999

    I didnt save that much money on the lab!

  3. 3
    Glen Davidson

    These days you don’t even need to be able to play sinister music on a pipe organ to be a mad scientist.

    Why do we let standards slip so badly?

    Glen Davidson

  4. 4
    Moggie

    Imagine if you attended the mad scientist workshop, and your nemesis was at the same workshop. That’d be awkward.

  5. 5
    wyobio

    This is stupid. Be trained in molecular biology in one week? Yeah, right, you’ll know how to troubleshoot when things don’t work, design proper controls, prep samples, analyze data etc. etc. I sure wasted 6 years on my PhD, should’ve bought $500 of equipment on ebay and spent $1400 on the workshop instead.

  6. 6
    a3kr0n

    I’m waiting until RepRap comes up with a home 3D protein printer/folder.

  7. 7
    ericpaulsen

    Can they teach me how to design a super-virus?

  8. 8
    EvoMonkey

    Are prominent facial scars required? Do you get an eye patch? How about Nehru collared lab coats?
    Does it include a class in developing an evil laugh – Bwahahahaha?

    So many things to think about in order to become a mad scientist.

  9. 9
    David Marjanović

    This workshop therefore combines opportunities to perform the latest, as well as commonly used older techniques, with particular attention to the chemical nuts and bolts behind them.

    In case anyone wonders, it’s true – the list contains techniques I’ve never even seen done, even though I did about half of the list myself during my bachelor’s in molecular biology.

  10. 10
    Erk12

    #5

    “I sure wasted 6 years on my PhD, should’ve bought $500 of equipment on ebay and spent $1400 on the workshop instead.”

    If all you acquired in your Ph.D. was the basic lab techniques of a moderately competent tech and a single run through the techniques, then I agree you wasted your time. I also would question the standards of the institution who granted your degree. No where do they promise or imply you will get the experience or abilities of a Ph.D. graduate. Did someone piss in your cereal this morning?

  11. 11
    marypoppins

    Did you miss the part that said:

    Primarily aimed at researchers who are new to the area, familiar but require a quick updating, or would like more practical bench training.

    ?

    Mary P

  12. 12
    gijoel

    Does the workshop cover elocution lessons on how to shout, “Fools I’ll show them all.”?

  13. 13
    marypoppins

    @ gijoel
    Maybe you can contact the modern language department?
    Mary P

  14. 14
    feralboy12

    They all laughed at me at the university. But who’s laughing now?

  15. 15
    Aquaria

    First a molecular biology lab–then take over the world!

    BWAHAHAHAHAHAHAHA

  16. 16
    gillt

    Various nucleic acid purification methodologies (silica bead, organic, and/or pI based), restriction digests, ligations, dephosphorylation assays, agarose gel electrophoresis, PCR, reverse transcriptase assay, real time qPCR, basic bioinformatics, (including blast tools)

    This covers almost everything you need to know for a next generation sequencing library prep. Cutting edge stuff!

  17. 17
    Francisco Bacopa

    You can extract pretty pure DNA with dish soap, salt, water, and rubbing alcohol.

  18. 18
    crys

    waaaait you’re saying I can do an RNA extraction and RT-PCR in my basement for $500?! I dont believe you!

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